GHEP - ISFG

Spanish and Portuguese-speaking Working Group of the International Society for Forensic Genetics

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mtDNA Canis lupus familiaris

van Asch B., Albarran C., Alonso A., Angulo R., Alves C., Betancor E., Catanesi C., Corach D., Crespillo M., Doutremepuich Ch. Estonba A., Fernandes A.T., Fernandez E., Garcia A.M., Garcia M.A., Gilardi P., Gonçalves R., Hernández A., Lima G., Nascimento E., M. de Pancorbo M., Parra D., Pinheiro M.F., Prat E., Puente J., Ramírez J.L., Rendo F., Rey I., Di Rocco F., Rodríguez A., Sala A., Salla J., Sanchez J.J., Solá D., Silva S., Pestano J.J., Amorim a,b. Forensic analysis of dog (Canis lupus familiaris) mitochondrial DNA sequences: An inter-laboratory study of the GEP-ISFG working group. For. Sci. Int. Genetics (2009) Vol. 4: 49-54.

A voluntary collaborative exercise aiming at the mitochondrial analysis of canine biological samples was carried out in 2006–2008 by the Non-Human Forensic GeneticsCommission of the Spanish and Portuguese Working Group (GEP) of the International Society for Forensic Genetics (ISFG). The participating laboratories were asked to sequence two dog samples (one blood stain and one hair sample) for the mitochondrial D-loop regioncomprised between positions 15,372 and 16,083 using suggested primers and PCR conditions, and to compare their results against a reference sequence.Twenty-one participating laboratories reported a total of 67.5% concordant results, 15% non-concordant results, and 17.5% no results. The hair sample analysis presented more difficulty to the participants than the bloodstain analysis, with a high percentage (29%) failing to obtain a result.The highlevel of participationshowedthe interest of thecommunity in theanalysis of dog forensic samples but the results reveal that crucial methodological issues need to be addressed and further training is required in order to respond proficiently to the demands of forensic casework.

GEP mtDNA Canis familiares. FSI Genet 2009